Agilent 1290

A binary pump combines two solvents to create a gradient typically using two independent pumps, each handling a specific solvent. These solvents are then mixed in a chamber after being pressurized by the pumps, resulting in a high-pressure mixing environment for the two solvents. Binary pumps are popular for HPLC applications, since high-pressure mixing has a lower gradient delay volume than quaternary pumps. This allows the mobile phase changes to reach the column faster. A traditional binary gradient uses an aqueous solvent/buffer as mobile phase A (pump A) and an organic solvent as mobile phase B (pump B). It is often used in high-throughput, high-resolution HPLC, UHPLC, or LC-MS applications.

A quaternary pump system uses a single pump to deliver the mobile phase, with gradient formation managed by a proportioning valve that mixes up to four solvents before they reach the pump head. Quaternary systems are very versatile with their wide flow range, high-pressure capabilities, and access to four solvents, making them suitable for various research, and method development applications.

The difference between a quaternary system and a binary system is that the quaternary system is mixed under low-pressure (low-pressure mixing), while the binary system is mixed under high-pressure (high-pressure mixing). The design of the quaternary pump requires slightly longer re-equilibration times compared to a binary system. Since the mixing occurs after the proportioning valve, a larger dwell volume than a binary pump can be observed. Quaternary and Binary systems also require the degassing of solvents before mixing. Online degassers are typically used for mobile phase degassing.

The flexible pump contains the high performance found within a high-pressure mixing UPLC pump with the greater flexibility of a low-pressure mixing HPLC pump. This pump is multipurpose as it has many additional functionalities including a mixer in/out switch, filter backflush, and automatic purge. The pump is also designed to resist high salt concentrations and wide pH gradients, making the pump ideal for mixing buffer solutions. Combined with the pump supporting all HPLC and UPLC methods, these functionalities make the flexible pump type one of the most versatile in the market.

The standard autosampler is a variable volume autosampler, which allows for samples of 1 ul up to 100 ul per injection. This large sample volume range combined with the 100-vial sample tray, makes this autosampler a versatile instrument for any laboratory.

The LC Injector HTC/HTS, with its high throughput injection of up to 100ul per injection and low carryover makes it ideal for its use in LC/MS. The LC injector HTS has 24 well plates which are temperature controlled, making it ideal for LC methods that require samples that are too volatile at room temperature. The LC Injector’s high throughput makes it ideal for large scale operations in laboratories.

The LC Injector HTC/HTS, with its high throughput injection of up to 100ul per injection and low carryover makes it ideal for its use in LC/MS. The LC injector HTS has 24 well plates which are temperature controlled, making it ideal for LC methods that require samples that are too volatile at room temperature. The LC Injector’s high throughput makes it ideal for large scale operations in laboratories.

The multisampler is a versatile autosampler which specializes in the handling of samples, with a capacity of up to 16 microplates and 6144 samples. The multisampler through its dual-needle design, is able to reduce wait times between cycles, which combined with its automated sample switching features, allows for its use in very high throughput injection methods. This makes the multisampler ideal in large scale lab operations.

The bio-inert multisampler is a versatile metal-free autosampler that specializes in the handling of biological samples, with a capacity of up to 16 microplates and 6144 samples. Through its dual-needle design, the multisampler can reduce wait times between, which combined with its automated sample switching features, allows for its use in very high throughput injection methods. The difference between the Bio-inert Multisampler and the Multisampler is that the sample flow path is metal-free. This enables the bio-inert multisampler to analyze biological samples such as proteins, nucleotides, and antibodies, making this multisampler ideal for medical and pharmaceutical laboratories.

The vialsampler is a versatile instrument that combines sample management with column handling. Its injection range of 0.1 to 100 uL, combined with a capacity to hold up to 132 2mL vials, or 36 6 mL vials allows this vial sampler to be one of the most versatile sample managers in a laboratory. The vialsampler differs from other autosamplers in that it can hold two columns, which can cool, or heat injected samples within a range of 5 to 80 °C, allowing for optimal management of many compounds. This makes this vial sampler one of the most effective sample managers in the market.

The Diode Array Detector (DAD FS) is used for integrating spectral acquisition into HPLC analyses. This detector’s use of spectral libraries, as well as multiple signal detection, and peak purity assessments allows for the enhancement of analytical qualification. The difference between the standard DAD versus the DAD FS is that the DAD FS uses a max-light cartridge cell, which is designed to have a light transmission of nearly 100% without reducing resolution due to cell-induced light dispersion. This gives the detector up to 10 times the sensitivity of a standard DAD flow cell, making this detector a great fit for methods requiring the highest degree of sensitivity, such as in pharmaceutical and medical research. ALPHA-1 recommends purchasing the inline pressure relief valve to protect the integrety of the flow cell against overpressurization.

The Diode Array Detector (DAD) is an essential instrument for integrating spectral acquisition into HPLC analyses. This detector’s use of spectral libraries, as well as multiple signal detection, and peak purity assessments allows for the enhancement of analytical qualification. This combined with a deuterium and tungsten dual-lamp system offers superior sensitivity, over a wide range from 190 nm to 950 nm. The DAD is also equipped with 1024 diodes and 1 nm slits, which provides exceptional spectral resolution for your analysis. The slits are also programmable, allowing for rapid sample characterization, which enables for the precise adjustments to optimize sensitivity and reduce baseline noise. These properties within the DAD makes it a versatile and high-performance tool for detailed spectral analysis.

The Diode Array Detector (DAD) is an essential instrument for integrating spectral acquisition into HPLC analyses. This detector’s use of spectral libraries, as well as multiple signal detection, and peak purity assessments allows for the enhancement of analytical qualification. This combined with a deuterium and tungsten dual-lamp system offers superior sensitivity, over a wide range from 190 nm to 950 nm. The DAD is also equipped with 1024 diodes and 1 nm slits, which provides exceptional spectral resolution for your analysis. The slits are also programmable, allowing for rapid sample characterization, which enables for the precise adjustments to optimize sensitivity and reduce baseline noise. These properties within the DAD makes it a versatile and high-performance tool for detailed spectral analysis.

The HPLC Variable Wavelength Detector (VWD) is designed to enhance detector sensitivity through its use of optical technology to reduce baseline drift and broaden linear range. The VWD can be used to facilitate the quantification of complex samples, which can include primary and secondary products, along with sample impurities. The VWD contains a deuterium lamp which provides optimal detection across a 190-600 nm wavelength, with a double beam photometer ensuring minimal baseline noise and stability. The VWD also offers programmable wavelength switching which allows for users to optimize sensitivity and selectivity to each analyte’s elution profile, as well as stop-flow wavelength scanning which allows for rapid wavelength optimization.

The HPLC Variable Wavelength Detector (VWD) is designed to enhance detector sensitivity through its use of optical technology to reduce baseline drift and broaden linear range. The VWD can be used to facilitate the quantification of complex samples, which can include primary and secondary products, along with sample impurities. The VWD contains a deuterium lamp which provides optimal detection across a 190-600 nm wavelength, with a double beam photometer ensuring minimal baseline noise and stability. The VWD also offers programmable wavelength switching which allows for users to optimize sensitivity and selectivity to each analyte’s elution profile, as well as stop-flow wavelength scanning which allows for rapid wavelength optimization.